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Science 17 January 1969:
Vol. 163. no. 3864, pp. 293 - 294
DOI: 10.1126/science.163.3864.293
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Articles

Sweetclover-Weevil Feeding Deterrent B: Isolation and Identification

W. R. Akeson 1, F. A. Haskins 1, and H. J. Gorz 1

1 Department of Agronomy, University of Nebraska, and Crops Research Division, Agricultural Research Service, U. S. Department of Agriculture, Lincoln, Nebraska 68503

Deterrent B, a compound apparently involved in the resistance of Melilotus infesta to the sweetclover weevil Sitona cylindricollis, has been isolated from leaves by a combination of preparative paper chromatography, sublimation, and crystallization. The compound has been identified as ammonium nitrate. Isolated deterrent B and ammonium nitrate have identical feeding deterrent activities. Although the deterrent principle was isolated as the ammonium salt, nitrate ion is probably responsible for the deterrent activity in vivo.

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Science. ISSN 0036-8075 (print), 1095-9203 (online)