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Science 17 January 1969:
Vol. 163. no. 3864, pp. 291 - 293
DOI: 10.1126/science.163.3864.291
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Articles

Sclerospora graminicola Axenic Culture

M. M. Tiwari 1 and H. C. Arya 1

1 Department of Botany, University of Rajasthan, Jaipur, India

Sclerospora graminicola (Sacc.) Schroet., the obligate pathlogen causing downy mildew (green-ear) of pearl millet (Pennisetum typhoides Stapf. and Hubb.) has been successfully cultured for the first time on a known semisynthetic mediumn with no evident loss of fructifications. Sclerospora graminicola was first grown on host callus tissue and subsequently on a modification of White's basal medium that contained casein hydrolyzate (Oxoid). 2,4-dichlorophenoxyacetic acid, and kinetin.

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Science. ISSN 0036-8075 (print), 1095-9203 (online)