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Science 17 January 1969:
Vol. 163. no. 3864, pp. 285 - 286
DOI: 10.1126/science.163.3864.285

Articles

Cytotoxic Test Automation: A Live-Dead Cell Differential Counter

Myron R. Melamed 1, Louis A. Kamentsky 2, and Edward A. Boyse 3

1 Memorial Hospital for Cancer and Allied Diseases, New York 10021
2 IBM Watson Laboratory, Columbia University, New York
3 Sloan-Kettering Institute, New York 10021

An appropriately modified cell spectrophotometer was used successfully for performing automatic counts of live and dead cells in the cytotoxic test, with trypan blue staining as an indicator of dead cells and light scattering to identify viable cells.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Quantitative single cell analysis and sorting.
P. Horan and L. Wheeless Jr (1977)
Science 198, 149-157
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Science. ISSN 0036-8075 (print), 1095-9203 (online)