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Science 29 November 1968:
Vol. 162. no. 3857, pp. 1024 - 1026
DOI: 10.1126/science.162.3857.1024
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Articles

Basis for the Acquaisition of Malignant Potential by Mouse Cells Cultivated in vitro

Stuart A. Aaronson 1 and George J. Todaro 1

1 Viral Carcinogenesis Branch, National Cancer Institute, Bethesda, Maryland 20014

Balb/c mouse embryo lines maintained in culture for over 200 generations under conditions that minimize cell-cell contact do not become tumorigenic. Lines cultivated under conditions where there is extensive cell contact become tumor-producing within 30 generations. The tissue-culture property that correlates best with tumorigenicity is the loss of contact inhibition of cell division.


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