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Science 8 November 1968:
Vol. 162. no. 3854, pp. 695 - 697
DOI: 10.1126/science.162.3854.695
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Articles

Lactate Dehydrogenase Isozymes: Dissociation and Denaturation by Dilution

Clement L. Markert 1 and Edward J. Massaro 1

1 Department of Biology, Yale University, New Haven, Connecticut 06520

The tetrameric enzyme lactate dehydrogenase dissociates into its constituent monomeric subunits in a high ion to protein concentration ratio, in certain ionic environments when frozen, and at extreme dilution. Dissociation by dilution involves changes in tertiary conformation which inactivate the enzyme. The dissociation is strongly inhibited by homologous native proteins, but only slightly by denatured or unrelated proteins.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Horseshoe Crab Lactate Dehydrogenase: Tissue Distribution and Molecular Weight.
E. J. Massaro (1970)
Science 167, 994-996
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Science. ISSN 0036-8075 (print), 1095-9203 (online)