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Science 1 November 1968:
Vol. 162. no. 3853, pp. 572 - 574
DOI: 10.1126/science.162.3853.572
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Articles

Isopycnic Separation of Subcellular Components from Poliovirus-Infected and Normal HeLa Cells

David Baltimore 1 and Alice S. Huang 1

1 Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139

A rapid method for determining the density of ribonucleoprotein particles and complexes has been developed. The method involves glutaraldehyde fixation of sucrose gradient fractions and immediate centrifugation for 5 hours through preformed cesium chloride gradients. There is little or no aggregation of particles, so that components which co-sediment in sucrose gradients are resolved by the cesium chloride gradient. By this method the densities of HeLa cell ribosomes, polyribosomes, and subribosomal particles have been determined. Furthermore, the poliovirus replication complex has been separated from polyribosomes and its density has been found to be unaffected by treatment with ethylene-diaminetetraacetic acid.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Transcription of Mitochondrial DNA in HeLa Cells.
G. Attardi, Y. Aloni, B. Attardi, D. Ojala, L. Pica-Mattoccia, D. L. Robberson, and B. Storrie (1970)
Cold Spring Harb Symp Quant Biol 35, 599-619
   Abstract »    PDF »
The Role of Ribosomal Subunits in Mammalian Cells.
C. Baglioni, C. Vesco, and M. Jacobs-Lorena (1969)
Cold Spring Harb Symp Quant Biol 34, 555-565
   Abstract »    PDF »
Regulation of Translation and Transcription of Messenger RNA during Early Embryonic Development.
L. H. Kedes, B. Hogan, G. Cognetti, S. Selvig, P. Yanover, and P. R. Gross (1969)
Cold Spring Harb Symp Quant Biol 34, 717-723
   Abstract »    PDF »
The Formation of Poliovirus Proteins.
D. Baltimore, M. F. Jacobson, J. Asso, and A. S. Huang (1969)
Cold Spring Harb Symp Quant Biol 34, 741-746
   Abstract »    PDF »


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Science. ISSN 0036-8075 (print), 1095-9203 (online)