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Science 13 September 1968:
Vol. 161. no. 3846, pp. 1157 - 1158
DOI: 10.1126/science.161.3846.1157
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Articles

Ruthenium Tetroxide for Fixing and Staining Cytoplasmic Membranes

P. Gaylarde 1 and I. Sarkany 1

1 Department of Dermatology, Royal Free Hospital, London, W.C.1

Rat liver and kidney were fixed for electron microscopy with ruthenium tetroxide. When compared with tissues fixed with osmiun tetroxide, the membranes showed well without additional staining. The trilaminar structuire of the cytoplasmic membranes was clearly seen. In width the nuclear, mitochondrial, and cytoplasmic membranes investigated resembled the cell memnbrane.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Microanalysis of Individual Mitochondrial Granules with Diameters Less Than 1000 Angstroms.
L. V. Sutfin, M. E. Holtrop, and R. E. Ogilvie (1971)
Science 174, 947-949
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Science. ISSN 0036-8075 (print), 1095-9203 (online)