Microspectrophotometry of Photoreceptor Organelles from Eyes of the Prawn Palaemonetes
Timothy H. Goldsmith 1,
Andrew E. Dizon 1, and
Hector R. Fernandez 1
1 Department of Biology, Yale University, New Haven, Connecticut
Microspectrophotometric measurements of individual dark-adapted rhabdoms of the prawn Palaemonetes vulgaris reveal the presence of two light-sensitive pigments. A pigment with maximum absorbancy at 555 nanometers is converted by light to a long-lived intermediate with wavelength of maximum absorbancy at 496 nanometers. A second pigment with wavelength of maximum absorbancy at 496 nanometers bleaches in the light, seemingly without forming detectable products at wavelengths longer than 375 nanometers. Both pigments occur in each layer of microvilli.