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Science 5 July 1968:
Vol. 161. no. 3836, pp. 59 - 60
DOI: 10.1126/science.161.3836.59
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Articles

Rat Mammary Gland Differentiation in vitro in the Absence of Steroids

William G. Dilley 1 and Satyabrata Nandi 1

1 Department of Zoology and Cancer Research Genetics Laboratory, University of California, Berkeley

Mammary glands from several strains of immature female rats will form small lobules of alveoli when cultured in chemically defined media lacking steroid hormones but containing insulin and prolactin. The degree of lobulo-alveolar differentiation is increased if estradiol, progesterone, and aldosterone are also included in the culture media.

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Science. ISSN 0036-8075 (print), 1095-9203 (online)