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Science 28 June 1968:
Vol. 160. no. 3835, pp. 1452 - 1454
DOI: 10.1126/science.160.3835.1452
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Articles

Pyrophosphate in the 5' Terminal Position of a Viral Ribonucleic Acid

Eckard Wimmer 1 and M. E. Reichmann 1

1 Department of Botany, University of Illinois, Urbana

A pancreatic ribonuclease digest of carbon-14-labeled Satellite Tobacco Necrosis Virus RNA was fractionated, according to charge, by column chromatography. Individual fractions were dephosphorylated with alkaline phosphomonoesterase and rechromatogramed. The fraction originally containing oligonucleotides with seven negative charges separated into two components corresponding to five and two negative charges, respectively, and therefore must have contained a terminal trinucleotide 5'-pyrophosphate, in addition to the internal hexanucleotides. Other fractions when similarly treated were found to contain only internal oligonucleotides.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
In vitro Synthesis of Phage RNA: The Nature of the Intermediates.
C. Weissmann, G. Feix, and H. Slor (1968)
Cold Spring Harb Symp Quant Biol 33, 83-100
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Science. ISSN 0036-8075 (print), 1095-9203 (online)