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Science 15 March 1968:
Vol. 159. no. 3820, pp. 1240 - 1242
DOI: 10.1126/science.159.3820.1240
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Articles

Ribosome Biogenesis: Nonrandom Addition of Structural Proteins to 50S Subunits

B. H. Sells 1 and F. C. Davis Jr. 1

1 Department of Biochemistry, St. Jude Hospital, and University of Tennessee, Memphis

Assemblage of structural proteins into 50S subunits was examined in Escherichia coli recovering fromt chloramphenicol treatment. Cells previously labeled with H3-leucine for three generations were incuibated for 30 minutes with chloramnlphenicol. Proteins synthesized during the initial 5 minuites of recovery from chlorarmphenicol treatmnent were labeled with C14-leucine. Marked variation in the ratios of C14- to H3-leucine in ribosomal protein occurred in cells that had been treated with chloramphenicol; luntreated cells displayed little variation. Thle resuilts sliggest that ribosomal proteins are assenmbled into 50S subunits in a nonrandom manner.

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Science. ISSN 0036-8075 (print), 1095-9203 (online)