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Science 15 March 1968:
Vol. 159. no. 3820, pp. 1239 - 1240
DOI: 10.1126/science.159.3820.1239
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Articles

Chaetopterus Photoprotein: Crystallization and Cofactor Requirements for Bioluminescence

Osamu Shimomura 1 and Frank H. Johnson 1

1 Department of Biology and Program in Biochemical Sciences, Princeton University, Princeton, New Jersey

The Chaetopterus photoprotein has been isolated in an amorphous form (molecular weight, 120,000) whlich in (NH4)2SO4 sollutionl converts to a crystalline form (molecular weight, 184,000) having the same specific light-emittinig activity; quantum yields are 0.0093 and 0.0143, respectively. Two new cofactor reqluirements have been separated fronm impure extracts: a macromnolecule resembling a nucleoprotein, and a lipid-like substance.

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Science. ISSN 0036-8075 (print), 1095-9203 (online)