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Science 1 March 1968:
Vol. 159. no. 3818, pp. 984 - 985
DOI: 10.1126/science.159.3818.984
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Articles

Respiratory Inhibition in Chlorella Produced by "Purified" Polyethylene Glycol 1540

H. Greenway 1, R. G. Hiller 2, and T. Flowers 2

1 Division of Irrigation Research, Commonwealth Scientific and Industrial Research Organization, Griffith, New South Wales
2 Department of Agricultural Sciences, Sutton Bonington, Loughborough, United Kingdom

Polyethylene glycol 1540, added to culture solution in amounts sufficient to reduce the water potential to -10 atmospheres, inhibited respiration in Chlorella much more than mannitol solutions at -10 atmospheres. This occurred despite the purification of the polyethylene glycol by passage through exchange columns. The toxic properties, which developed some time after purification, increased with time of storage of solutions of polyethylene glycol 1540 at room temperature.

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Science. ISSN 0036-8075 (print), 1095-9203 (online)