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Science 2 February 1968:
Vol. 159. no. 3814, pp. 540 - 542
DOI: 10.1126/science.159.3814.540
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Articles

Phytostat for Continuous Culture and Automatic Sampling of Plant-Cell Suspensions

R. A. Miller 1, J. P. Shyluk 1, O. L. Gamborg 1, and J. W. Kirkpatrick 1

1 Prairie Regional Laboratory, National Research Council of Canada, Saskatoon, Saskatchewan

An apparatus for growing plant cells in suspension culture is described; it may be used for continuous or batch culture, and is equipped with a valve for automatic collection of samples. Aeration is by continuous bubbling of air into the culture through fritted glass. Normal culture-duplication times are from 30 to 35 hours.

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Science. ISSN 0036-8075 (print), 1095-9203 (online)