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Science 19 January 1968:
Vol. 159. no. 3812, pp. 312 - 314
DOI: 10.1126/science.159.3812.312
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Articles

Visual Pigment Fluorescence

Anthony V. Guzzo 1 and Gary L. Pool 1

1 University of Wyoming, Laramie

The fluorescence of cattle rod outer segments (dried) and of rhodopsin in solution lies in the range of 575 to 600 millimicrons with a quantum efficiency of 0.005 if excitation is in the visible band near 500 millimicrons. The emission is abolished by bleaching at -196°C but can be reversibly regenerated by irradiation with light of longer wavelength (600 millimicrons). This behavior reflects the known interconversion of rhodopsin to prelumirhodopsin at this temperature.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Fluorescence of photoreceptor cells observed in vivo.
N Franceschini, K Kirschfeld, and B Minke (1981)
Science 213, 1264-1267
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