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Science 22 December 1967:
Vol. 158. no. 3808, pp. 1575 - 1577
DOI: 10.1126/science.158.3808.1575
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Articles

Growth of Isolated Mesophyll Cells of Arachis hypogaea in Simple Defined Medium in vitro

P. C. Joshi 1 and G. R. Noggle 1

1 Department of Botany, North Carolina State University, Raleigh 27607

Isolated mesophyll cells from leaflets of Arachis hypogaea can be cultured in a simple, defined liquid nutrient medium containing minerals, with an appropriate source of ammonia, sugar, 2,4-dichlorophenoxyacetic acid, and kinetin. The significance of such a simple medium in understanding the problems of cell metabolism, growth, and morphogenesis is discussed.


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