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Science 20 October 1967:
Vol. 158. no. 3799, pp. 385 - 387
DOI: 10.1126/science.158.3799.385
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Articles

Sulfhydryl Groups and Estradiol-Receptor Interaction

Elwood V. Jensen 1, Daniel J. Hurst 1, Eugene R. DeSombre 1, and Peter W. Jungblut 1

1 Ben May Laboratory for Cancer Research, University of Chicago, Illinois 60637

The characteristic ability of rat uteri to take up tritiated estradiol in vitro or to retain estradiol previously incorporated either in vivo or in vitro is destroyed by treating the tissue with various sulfhydryl-blocking reagents. The two radioactive estradiol-receptor complexes, observed in uterine homogenates in the supernatant fraction and in an extract of the nuclear fraction, respectively, are disrupted by brief exposure to organic mercurials in the cold. Sulfhydryl groups of uterine receptor substances apparently play a vital role in estradiol binding, perhaps indirectly through contribution to receptor conformation.


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