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Science 11 August 1967:
Vol. 157. no. 3789, pp. 709 - 711
DOI: 10.1126/science.157.3789.709
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Articles

Cytoplasmic and Chloroplast Ribosomes of Chlamydomonas: Ultracentrifugal Characterization

Ruth Sager 1 and Mary G. Hamilton 2

1 Department of Biological Sciences, Hunter College, New York
2 Sloan-Kettering Division, Graduate School of Medical Sciences, Cornell University Medical College, New York 10021

Ribosomes isolated from the cytoplasmic and chloroplast fractions of Chlamydomonas were characterized in the ultracentrifuge. The cytoplasmic ribosomes belong to the 80S class of ribosomes, and, like animal ribosomes, dissociate to 60, 50, and 40S subunits. However, like the ribosomes of microorganisms, they contain smaller RNA's, 24 and 16S, and require 0.01 mole of magnesium ions per liter for stability. Chloroplast ribosomes are 70S like those of higher plants but are very unstable. A stable 50S subunit has been observed.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Proteomic Characterization of the Chlamydomonas reinhardtii Chloroplast Ribosome: IDENTIFICATION OF PROTEINS UNIQUE TO THE 70 S RIBOSOME.
K. Yamaguchi, M. V. Beligni, S. Prieto, P. A. Haynes, W. H. McDonald, J. R. Yates III, and S. P. Mayfield (2003)
J. Biol. Chem. 278, 33774-33785
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Science. ISSN 0036-8075 (print), 1095-9203 (online)