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Science 19 May 1967:
Vol. 156. no. 3777, pp. 949 - 951
DOI: 10.1126/science.156.3777.949
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Articles

Fluorescence Decay Times: Proteins, Coenzymes, and Other Compounds in Water

Raymond F. Chen 1, Gerald G. Vurek 1, and Nelson Alexander 1

1 National Heart Institute, Bethesda, Maryland 20014

The fluorescence decay time (tgr) was 2 to 5 nanoseconds for proteins and 4 to 5 nanoseconds for flavin, pyridine nucleotide, and vitamin B6coenzymes; tgr varied widely in 48 compounds measured in water. Altholugh reported values of tgr for a few of the soluttions studied were in excellenlt agreement. previously "calculated" lifetimes, in severall instances, are apparently erroneous. Nonexponential decay was detectable with our "nanosecond-flash" apparatus, a modification of the first commercially aavilable unit for determination of tgr.

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Science. ISSN 0036-8075 (print), 1095-9203 (online)