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Science 21 April 1967:
Vol. 156. no. 3773, pp. 400 - 401
DOI: 10.1126/science.156.3773.400
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Malic Dehydrogenase Isozymes: Distribution in Developing Nucleate and Anucleate Halves of Sea Urchin Eggs

Grant W. Patton Jr. 1, Laurens Mets 1, and Claude A. Villee 1

1 Department of Biological Chemistry, Harvard Medical School, Boston, Massachusetts

Nucleate and anucleate halves of sea urchin eggs have seven and five forms of L-malic dehydrogenase, respectively. Fertilization results in reduction of the number of enzyme forms in both halves. The normal complement of seven isozymes in unfertilized eggs appears to be a synthesis of five soluble and four particulate forms. Reduction in the number of isozymes after fertilization takes place principally in solutble forms and appears to be under cytoplasmic control.

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Science. ISSN 0036-8075 (print), 1095-9203 (online)