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Science 14 April 1967:
Vol. 156. no. 3772, pp. 256 - 257
DOI: 10.1126/science.156.3772.256

Articles

Artifact Produced in Disc Electrophoresis by Ammonium Persulfate

John M. Brewer 1

1 Department of Biochemistry, University of Georgia, Athens 30601

Ammonium persulfate, a common polymerizing agent for acrylamide gels, can inactivate yeast enolase and produce increased electrophoretic heterogeneity during disc electrophoresis in gels containing 8M urea. The use of riboflavin and light for polymerization or thioglycolate for removal of the persulfate are feasible alternatives.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Polyacrylamide Gel Electrophoresis.
A. Chrambach and D. Rodbard (1971)
Science 172, 440-451
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Science. ISSN 0036-8075 (print), 1095-9203 (online)