Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Science 10 March 1967:
Vol. 155. no. 3767, pp. 1271 - 1273
DOI: 10.1126/science.155.3767.1271
Prev | Table of Contents | Next

Articles

Functional Chloroplast Polyribosomes from Tobacco Leaves

Jane L. Chen 1 and S. G. Wildman 1

1 Department of Botanical Sciences and Molecular Biology Institute, University of California, Los Angeles

Incubation of isolated to bacco chloroplasts with ingredients re quired for protein synthesis resulted in liberation of 70S ribosomnes and poly ribosomes that no longer sedimented with the chloroplasts. With increasing time of incubation, polyribosomes broke down to 70S monosomes. Similarly, microgram quantities of ribonuclease caused chloroplast polyribosomes to break down into monosomes. Both polyribosomes and 70S ribosomes that were isolated on sucrose density gra dients and tested separately in cell-free systems were capable of protein syn thesis; however, polyribosomes formed more protein per unit of RNA than monosomes did.

To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)