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Science 17 February 1967:
Vol. 155. no. 3764, pp. 835 - 836
DOI: 10.1126/science.155.3764.835
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Articles

Leukocyte Oxidase: Defective Activity in Chronic Granulomatous Disease

Robert L. Baehner 1 and David G. Nathan 1

1 Department of Medicine, Children's Hospital Medical Center, and Harvard Medical School, Boston, Massachusetts

The intact leukocytes of two children with chronic granulomatous disease fail to reduce nitroblue tetrazolium during phagocytosis. This is due to defective operation of an oxidase of reduced nicotinamide adenine dinucleotide that is insensitive to cyanide and that indirectly stimulates the oxidation of glucose-6-phosphate in leukocytes. Such leukocytes undergo no increase in oxygen consumption or in activity of the hexose monophosphate shunt during phagocytosis, although lactate production is normal. The addition of nitroblue tetrazolium to a leukocyte suspension appears to provide a sensitive diagnostic screening test for this disease.


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Chronic Granulomatous Disease 1974.
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Use of the Nitro Blue Tetrazolium Test in Clinical Pediatrics.
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