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Science 3 February 1967:
Vol. 155. no. 3762, pp. 575 - 577
DOI: 10.1126/science.155.3762.575
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Articles

Genomic Exclusion: A Rapid Means for Inducing Homozygous Diploid Lines in Tetrahymena pyriformis, Syngen 1

Sally Lyman Allen 1

1 Department of Zoology, University of Michigan, Ann Arbor

Genomic exclusion is an abnormal form of conjugation occurring between cells with defective micronuclei and normal cells with diploid micronuclei. The progeny are heterocaryons; each cell has an old macronucleus but a new diploid micronucleus derived from one meiotic product of the normal mate. Such cells express genes found in the old macronucleus, are sexually mature, and can be specifically selected. When inbred, they give rise to lines genetically homozygous at all known loci.


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Deletion of the Tetrahymena thermophila rDNA replication fork barrier region disrupts macronuclear rDNA excision and creates a fragile site in the micronuclear genome.
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Analysis of a Mutant Exhibiting Conditional Sorting to Dense Core Secretory Granules in Tetrahymena thermophila.
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High Frequency Intragenic Recombination During Macronuclear Development in Tetrahymena thermophila Restores the Wild-type SerH1 Gene.
J. C. Deak and F. P. Doerder (1998)
Genetics 148, 1109-1115
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Germ-line knockout heterokaryons of an essential alpha -tubulin gene enable high-frequency gene replacement and a test of gene transfer from somatic to germ-line nuclei in Tetrahymena thermophila.
B. Hai and M. A. Gorovsky (1997)
PNAS 94, 1310-1315
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DNA Termini in Ciliate Macronuclei.
E.H. Blackburn, M.L. Budarf, P.B. Challoner, J.M. Cherry, E.A. Howard, A.L. Katzen, W.-C. Pan, and T. Ryan (1983)
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Osmotic shock prevents nuclear exchange and produces whole-genome homozygotes in conjugating Tetrahymena.
E Orias, E. Hamilton, and M Flacks (1979)
Science 203, 660-663
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Science. ISSN 0036-8075 (print), 1095-9203 (online)