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Science 6 January 1967:
Vol. 155. no. 3758, pp. 97 - 99
DOI: 10.1126/science.155.3758.97
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Articles

Negro Variant of Glucose-6-Phosphate Dehydrogenase Deficiency (A-) in Man

Akira Yoshida 1 and Arno G. Motulsky 2

1 George Stamatoyannopoulos
2 Division of Medical Genetics, Department of Medicine, University of Washington, Seattle

Glucose-6-phosphate dehydrogenase in erythrocytes of the Negro type associated with enzyme deficiency (A-) was separated by chromatography on a carboxymethyl-Sephadex column from the electrophoretically indistinguishable Negro variant with normal enzyme activity (A+). Quantitative immunologic neutralization tests indicated that the A- enzyme had about the same enzymatic and serological activity as the A+ and the normal (B+) enzymes. The enzyme activity of the A- variant in young erythrocytes was similar to that in young cells from normal individuals, although the activity of the A- variant in unfractionated red cells was 10 to 15 percent of normal. These data indicate that the basic defect in the variant enzyme (A-) is a structural mutation which causes more rapid degradation of the enzyme during erythrocyte aging.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Metachromatic Leukodystrophy (MLD) X. Immunological Studies of the Abnormal Sulfatase A.
D. Stumpf, E. Neuwelt, J. Austin, and P. Kohler (1971)
Arch Neurol 25, 427-431
   Abstract »    PDF »
Athens Variant of Glucose-6-Phosphate Dehydrogenase.
G. Stamatoyannopoulos, A. Yoshida, Chr. Bacopoulos, and A. G. Motulksy (1967)
Science 157, 831-833
   Abstract »    PDF »


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