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Science 16 December 1966:
Vol. 154. no. 3755, pp. 1463 - 1464
DOI: 10.1126/science.154.3755.1463
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Articles

Centromere: Absence of DNA Replication during Chromatid Separation in Human Fibroblasts

David E. Comings 1

1 Department of Medical Genetics, City of Hope Medical Center, Duarte, California

Cultures of human fibroblasts were labeled briefly with tritiated thymidine and fixed; autoradiographs were made and exposed for 3frac12 months. No labeling was noted over the centromere of metaphase or anaphase chromosomes. The technique was sensitive to replication at the centromere of a DNA helix only 2.5 microns long, considerably shorter than the estimated length of a replicon in humans. This suggests that chromatid separation during mitosis is not associated with delayed replication of a short segment of chromosomal DNA.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Splitting the Chromosome: Cutting the Ties That Bind Sister Chromatids.
K. Nasmyth, J. Peters, and F. Uhlmann (2000)
Science 288, 1379-1384
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