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Science 25 November 1966:
Vol. 154. no. 3752, pp. 1027 - 1029
DOI: 10.1126/science.154.3752.1027
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Articles

Co-linearity of beta-Galactosidase with Its Gene by Immunological Detection of Incomplete Polypeptide Chains

Audree V. Fowler 1 and Irving Zabin 1

1 Department of Biological Chemistry, University of California School of Medicine, and Molecular Biology Institute, Los Angeles 90024

Many nonsense mutants that map in the beta-galactosidase structural gene produce material that forms precipitin lines when tested by double diffusion on agar against antiserum prepared from native beta-galactosidase. Relative sizes of the cross-reacting materia!s measured by sucrose density gradient centrifugation are the same as sizes calculated from genetic mapping of nonsense mutants. Orientation of the protein to its gene is also indicated.


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