Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Science 4 November 1966:
Vol. 154. no. 3749, pp. 670 - 671
DOI: 10.1126/science.154.3749.670
Prev | Table of Contents | Next

Articles

Deoxyribonucleic Acid in Germinal Vesicles of Oocytes of Rana pipiens

Alex J. Haggis 1

1 Department of Biology, Reed College, Portland, Oregon

The amount of DNA isolated from germinal vesicles of Rana pipiens oocytes is similar to that isolated from whole unfertilized eggs, and this suggests that oocyte nuclei are the source ofmuch of the DNA obtained from whole eggs. Fluorometric determinations of the isolated DNA show the presence of 0.004 microgram of DNA equivalents per germinal vesicle. Similar values were obtained by estimations from comparisons of the cesium chloride densitygradient profiles of sample DNA and a known amount of reference DNA from Pseudomonas aeruginosa. The buoyant density of DNA prepared from isolated germinal vesicles is the same as that of frog-liver DNA, 1.706 grams per cubic centimeter, as determined by equilibrium sedimentation in a cesium chloride density gradient.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Specific Gene Amplification in Oocytes: Oocyte nuclei contain extrachromosomal replicas of the genes for ribosomal RNA.
D. D. Brown and I. B. Dawid (1968)
Science 160, 272-280
   PDF »


To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)