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Science 9 September 1966:
Vol. 153. no. 3741, pp. 1269 - 1271
DOI: 10.1126/science.153.3741.1269
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Articles

Chloroplast DNA from Tobacco Leaves

K. K. Tewari 1 and S. G. Wildman 1

1 Department of Botany and Plant Biochemistry and the Molecular Biology Institute, University of California, Los Angeles 90024

DNA from tobacco leaf chloroplasts was isolated as a single component with a buoyant density in CsCl of 1.702 compared to 1.697 for nuclear DNA. 5-Methylcytosine is present in nuclear DNA but absent in chloroplast DNA. Chloroplast DNA, with a guanine-cytosine content of 43 percent, has a melting temperature of 86°C and renatures completely on slow cooling, whereas nuclear DNA ( melting temperature, 84°C; guanine-cytosine content, 40 percent) does not renature. About 9 percent of the total DNA in tobacco leaves is chloroplast DNA representing about 4.7 xX 10-15 gram of DNA per chloroplast with a molecular weight of approximately 4 xX 107.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
5-Methylcytosine in eukaryotic DNA.
M Ehrlich and R. Wang (1981)
Science 212, 1350-1357
   Abstract »    PDF »
Chloroplast Replication and Growth in Tobacco.
R. Boasson and W. M. Laetsch (1969)
Science 166, 749-751
   Abstract »    PDF »


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