Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Science 29 July 1966:
Vol. 153. no. 3735, pp. 545 - 547
DOI: 10.1126/science.153.3735.545
Prev | Table of Contents | Next

Articles

One-Way Stimulation in Mixed Leukocyte Cultures

Fritz H. Bach 1 and Nancy K. Voynow 1

1 Departments of Medical Genetics and Medicine, University of Wisconsin, Madison

We have developed an improved method for the mixed leukocyte culture test. Control values, as determined by rates of incorporation of thymidine, are very low, allowing evaluation of low levels of stimulation in homologous cell mixtures. One-way stimulation is assayed by treating the cells of one individual with mitomycin C; treated cells cannot respond (incorporate thymidine) but can still stimulate homologous untreated cells to do so.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Long-Term Cardiac Allograft Survival across an MHC Mismatch after "Pruning" of Alloreactive CD4 T Cells.
M. Hu, D. Watson, G. Y. Zhang, N. Graf, Y. M. Wang, M. Sartor, B. Howden, J. Fletcher, and S. I. Alexander (2008)
J. Immunol. 180, 6593-6603
   Abstract »    Full Text »    PDF »
HLA Typing for Bone Marrow Transplantation: New Polymerase Chain Reaction--Based Methods.
A. B. Begovich and H. A. Erlich (1995)
JAMA 273, 586-591
   Abstract »    PDF »
Chronic Idiopathic Thrombocytopenic Purpura: A Familial Immunodeficiency Syndrome?.
M. J. Stuart, R. H. Tomar, M. L. Miller, and F. R. Davey (1978)
JAMA 239, 939-942
   Abstract »    PDF »
Phenytoin-Induced Pseudolymphoma Syndrome: An Immunologic Study.
E. N. Charlesworth (1977)
Arch Dermatol 113, 477-480
   Abstract »    PDF »
Phagocyte Function and Cell-Mediated Immunity in Systemic Lupus Erythematosus.
M. Landry (1977)
Arch Dermatol 113, 147-154
   Abstract »    PDF »
Immune Reconstitution in Keratoconjunctivitis and Superficial Candidiasis: The Role of Immunocompetent Lymphocyte Transfusion and Transfer Factor.
V. G. Wong and C. H. Kirkpatrick (1974)
Arch Ophthalmol 92, 335-339
   Abstract »    PDF »
Preferential Cutaneous Infiltration by Neoplastic Thymus-Derived Lymphocytes: Morphologic and Functional Studies.
R. L. EDELSON, C. H. KIRKPATRICK, E. M. SHEVACH, P. S. SCHEIN, R. W. SMITH, I. GREEN, and M. LUTZNER (1974)
Ann Intern Med 80, 685-692
   Abstract »    PDF »
Erythema Nodosum Following Gold Sodium Thiomalate Therapy.
R. L. Stone, A. Claflin, and N. S. Penneys (1973)
Arch Dermatol 107, 602-604
   Abstract »    PDF »
Diseases of Cellular Immunity.
E. R. STIEHM, A. J. AMMANN, E. V. BARNETT, C. G. CRADDOCK, H. H. FUDENBERG, and G. J. LAWLOR JR. (1972)
Ann Intern Med 77, 101-116
   Abstract »    PDF »
Genetic and Immunological Complexity of Major Histocompatibility Regions.
F. H. Bach, M. B. Widmer, M. Segall, M. L. Bach, and J. Klein (1972)
Science 176, 1024-1027
   Abstract »    PDF »
Liver Histiocytes in Rejection of Tumor Cells: Preliminary Studies With the Isolated, Perfused Rodent Liver.
S. Draz, J. E. Byfield, P. Byfield, and E. W. Fonkalsrud (1971)
Arch Surg 102, 574-577
   Abstract »    PDF »
Immunosuppression and the Guillain-Barre Syndrome.
D. A. Drachman, P. Y. Paterson, B. S. Berlin, and J. Roguska (1970)
Arch Neurol 23, 385-393
   Abstract »    PDF »
Long-Term Integrity of Renal Function in Cadaver Allografts.
W. D. Shelp, F. H. Bach, W. A. Kisken, M. Newton, R. E. Rieselbach, and A. B. Weinstein (1970)
JAMA 213, 1443-1447
   Abstract »    PDF »
Transplantation: Pairing of Donor and Recipient.
F. H. Bach (1970)
Science 168, 1170-1178
   PDF »
The Gastric Disorder in Immunoglobulin-Deficient Patients.
J. J. TWOMEY, P. H. JORDAN JR., A. H. LAUGHTER, H. J. MEUWISSEN, and R. A. GOOD (1970)
Ann Intern Med 72, 499-504
   Abstract »    PDF »
Leukemia-associated antigens in the mixed leukocyte culture test..
M. L. Bach, F. H. Bach, and P. Joo (1969)
Science 166, 1520-1522
   Abstract »    PDF »
Modification of Renal Allograft Rejection in Man.
C. B. Carpenter and J. P. Merrill (1969)
Arch Intern Med 123, 501-513
   Abstract »    PDF »
The Preoperative Assessment of Histocompatibility.
C. M. Zmijewski and J. L. Fletcher (1969)
Arch Intern Med 123, 514-520
   Abstract »    PDF »
"Acquired" Agammaglobulinemia with Defective Delayed Hypersensitivity.
W. L. KOPP, J. S. TRIER, E. R. STIEHM, and P. FOROOZAN (1968)
Ann Intern Med 69, 309-317
   Abstract »    PDF »
Transplantation: Problems of Histocompatability Testing: Typing and matching tests are useful in pairing donors and recipients for organ transplantation.
F. H. Bach (1968)
Science 159, 1196-1198
   PDF »
Hu-1: Major Histocompatibility Locus in Man.
F. H. Bach and D. B. Amos (1967)
Science 156, 1506-1508
   Abstract »    PDF »
Mixed Leukocyte Reactions and Histocompatibility in Rats.
W. K. Silvers, D. B. Wilson, and J. Palm (1967)
Science 155, 703-704
   Abstract »    PDF »


To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)