Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Science 22 April 1966:
Vol. 152. no. 3721, pp. 521 - 523
DOI: 10.1126/science.152.3721.521
Prev | Table of Contents | Next

Articles

Biosynthesis and Composition of Histones in Novikoff Hepatoma Nuclei and Nucleoli

Lubomir S. Hnilica 1, Ming C. Liau 1, and Robert B. Hurlbert 1

1 Department of Biochemistry, University of Texas M. D. Anderson Hospital and Tumor Institute, 6723 Bertner Avenue, Houston 77025

Histones were prepared from isolated nuclei and nucleoli of the Novikoff ascitic hepatoma at several time points after the injection of L-lysine uniformly labeled with C14 into tumor-bearing rats. Amino acid analysis and starch-gel electrophoresis failed to reveal any differences between the nuclear and nucleolar histones, although both fractions were more acidic in composition than calf thymus histones. However, the nucleolar histones were a metabolically distinct fraction, and their rate of synthesis was approximately twice that of the total nuclear histones.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Histones in the Wild-Type and the Anucleolate Mutant of Xenopus laevis.
L. Berlowitz and M. L. Birnstiel (1967)
Science 156, 78-80
   Abstract »    PDF »


To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)