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Science 11 February 1966:
Vol. 151. no. 3711, pp. 689 - 691
DOI: 10.1126/science.151.3711.689
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Articles

Organization of DNA in Dipteran Polytene Chromosomes as Indicated by Polarized Fluorescence Microscopy

James W. MacInnes 1 and Robert B. Uretz 1

1 Department of Biophysics, University of Chicago, Chicago, Illinois

Analysis of the polarization of fluorescence from Drosophila virilis polytene chromosomes stained with acridine orange suggests that the DNA in the interband regions of these chromosomes cannot be in a supercoiled configuration, but must lie parallel to the chromosome axis.


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