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Science 3 September 1965:
Vol. 149. no. 3688, pp. 1098 - 1099
DOI: 10.1126/science.149.3688.1098
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Articles

Lability of Host-Cell DNA in Growing Cell Cultures Due to Mycoplasma

Charles C. Randall 1, Lanelle G. Gafford 1, Glenn A. Gentry 1, and Lucy A. Lawson 1

1 Department of Microbiology, School of Medicine, University of Missisvsippi, Jackson

In HeLa-cell cultures chronically infected with Mycoplasma, (PPLO) host-cell DNA is unstable as detected by incorporation of H3-or C1 i-thymidine into DNA and subsequent release into the medium as acidsoluble radioactivity. This characteristic can be transmitted to PPLO-free cultures of strain L cells by inoculation with preparations of PPLO from the HeLa cells, although chronically infected cultures of L cells continue to multiply. In addition, virus preparations also may carry PPLO contamination through numerous passages.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Shear Sensitivity of the E. coli Genome: Multiple Membrane Attachment Points of the E. coli DNA.
B. H. Rosenberg and L. F. Cavalieri (1968)
Cold Spring Harb Symp Quant Biol 33, 65-72
   Abstract »    PDF »
Chromosome Changes Induced by Infections in Tissues of Rhynchosciara angelae.
C. Pavan and R. Basile (1966)
Science 151, 1556-1558
   Abstract »    PDF »


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