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Science 3 September 1965:
Vol. 149. no. 3688, pp. 1096 - 1098
DOI: 10.1126/science.149.3688.1096
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Articles

Inhibition of Protein Synthesis by Spectinomycin

Julian Davies 1, Porter Anderson 1, and Bernard D. Davis 1

1 Department of Bacteriology and Immunology, Harvard Medical School, Boston, Massachusetts

Spectinomycin selectively inhibits protein synthesis in cells and in extracts of Escherichia coli. Mutations to high-level resistance to this antibiotic map close to the streptomycin locus, and the site of action of spectinomycin, like that of streptomycin, is the 30S ribosomal subunit, as shown by experiments with reconstituted 70S ribosomes containing subunits from sensitive and from resistant ribosomes. In contrast to streptomycin, however, spectinomycin is not bactericidal and causes no detectable misreading of polyribonucleotides.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
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J. Bacteriol. 182, 3896-3903
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Linkage Map of Escherichia coli K-12, Edition 10: The Traditional Map.
M. K. B. Berlyn (1998)
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Spectinomycin-Resistant Neisseria gonorrhoeae.
C. Thornsberry, H. Jaffee, S. T. Brown, T. Edwards, J. W. Biddle, and S. E. Thompson (1977)
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Drugs Five Years Later: Spectinomycin.
W. M. McCORMACK and M. FINLAND (1976)
Ann Intern Med 84, 712-716
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The Chemical and Genetic Structure of Bacterial Ribosomes.
P. S. Sypherd, D. M. O'Neil, and M. M. Taylor (1969)
Cold Spring Harb Symp Quant Biol 34, 77-84
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Altered Ribosomes in Antibiotic-Resistant Mutants of E. coli.
A. Bollen, T. Helser, T. Yamada, and J. Davies (1969)
Cold Spring Harb Symp Quant Biol 34, 95-100
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Approaches to the Genetics of Escherichia coli Ribosomes.
D. Apirion, S. L. Phillips, and D. Schlessinger (1969)
Cold Spring Harb Symp Quant Biol 34, 117-128
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Escherichia coli: High Resistance or Dependence on Streptomycin Produced by the Same Allele.
L. Luzzatto, D. Schlessinger, and D. Apirion (1968)
Science 161, 478-479
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Mutations and Genetics Concerned With the Ribosome.
J. G. Flaks, P. S. Leboy, E. A. Birge, and C. G. Kurland (1966)
Cold Spring Harb Symp Quant Biol 31, 623-631
   Abstract »    PDF »
Single-Stranded DNA as a Template for in Vitro Protein Synthesis.
B. J. McCarthy, J. J. Holland, and C. A. Buck (1966)
Cold Spring Harb Symp Quant Biol 31, 683-691
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