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Science 27 August 1965:
Vol. 149. no. 3687, pp. 983 - 985
DOI: 10.1126/science.149.3687.983
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Articles

Hemadsorption-Negative Plaque Test: New Assay for Rubella Virus Revealing a Unique Interference tion-negative plaque technique

Philip I. Marcus 1 and David H. Carver 2

1 Department of Microbiology and Immunology
2 Departments of Microbiology and Immunology and Pediatrics, Albert Einstein College of Medicine, Bronx, New York, 10461

A simple and rapid plaque procedure has been developed for detecting and accurately assaying rubella virus in a noncytopathic virus-cell relationship. Plaque-formation is based on the development, in individual cells infected with rubella virus, of a unique type of intrinsic interference to infection with Newcastle disease virus. Rubella virus—infected cells challenged with Newcastle disease virus and tested for hemadsorption 15 hours later stand out as hemadsorption-negative areas. Individual living cells infected with rubella virus can be resolved under conditions allowing standard cloning procedures. In principle, the hemadsorption-negative plaque test can be used to search for a new class of noncytopathic, non-hemadsorbing viruses—those that induce an intrinsic interference to infection by any hemadsorbing virus.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Production of Hemadsorption-Negative Areas by Sera Containing Australia Antigen.
D. H. Carver and D. S. Y. Seto (1972)
Arch Pediatr Adolesc Med 123, 413-415
   Abstract »    PDF »
Interferon Action: Inhibition of Vesicular Stomatitis Virus RNA Synthesis Induced by Virion-Bound Polymerase.
P. I. Marcus, D. L. Engelhardt, J. M. Hunt, and M. J. Sekellick (1971)
Science 174, 593-598
   Abstract »    PDF »
Production of Hemadsorption-Negative Areas by Serums Containing Australia Antigen.
D. H. Carver and D. S. Y. Seto (1971)
Science 172, 1265-1267
   Abstract »    PDF »
Direct Hemadsorption by Cell Cultures Infected With Rubella Virus.
C. A. Perlino and P. Isacson (1969)
Arch Pediatr Adolesc Med 118, 83-88
   Abstract »    PDF »
Antigens of Rubella Virus.
N. J. Schmidt and E. H. Lennette (1969)
Arch Pediatr Adolesc Med 118, 89-93
   Abstract »    PDF »
Viral Inhibition of Lymphocyte Response to Phytohemagglutinin.
J. R. Montgomery, M. A. South, W. E. Rawls, J. L. Melnick, G. B. Olson, P. B. Dent, and R. A. Good (1967)
Science 157, 1068-1070
   Abstract »    PDF »


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