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Science 30 April 1965:
Vol. 148. no. 3670, pp. 638 - 640
DOI: 10.1126/science.148.3670.638
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Articles

Rotation Technique in Electron Microscopy of Viruses

Hari Om Agrawal 1, J. W. Kent 2, and D. M. MacKay 3

1 Department of Virology, State Agricultural University, Wageningen, Holland
2 Weed Control Department, Institute for Biological and Chemical Research on Field Crops and Herbage, Wageningen, Holland
3 Department of Communication, University of Keele, Staffordshire, England

Some of the features revealed by the rotation technique of examining electron micrographs may be artifacts. To avoid misinterpretation, "rotated" photographs should be compared with originals. The criterion of reinforcement of structures in the "rotated" photographs is not enough, and it cannot be presumed that all the structures reinforced are actually present in the object under study. Further tests for radial symmetry in the arrangement of elements in a pattern should be made.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Rotation Technique in Radially Symmetric Electron Micrographs: Mathematical Analysis.
R. S. Norman (1966)
Science 152, 1238-1239
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