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Science 23 April 1965:
Vol. 148. no. 3669, pp. 519 - 521
DOI: 10.1126/science.148.3669.519
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Articles

Control of Glutamine Synthetase in the Embryonic Retina in vitro

A. A. Moscona 1 and David L. Kirk 1

1 Department of Zoology, University of Chicago, Chicago, Illinois 60637

Glutamine synthetase activity in the neural retina of the chick embryo increases sharply during terminal differentiation of this tissue. This characteristic increase can be reproduced in cultures of retinal tissue fragments from late embryos. A similarly sharp increase can be elicited precociously in younger retina by culturing in medium with adult serum. Both the precociously elicited and the later increase in enzyme activity require continuous protein synthesis; both can be suppressed during the first 24 hours of culture by blocking RNA synthesis or by removing the adult serum. Subsequently, the increase in enzyme activity becomes progressively less dependent on RNA synthesis and on the continuous presence of adult serum. This transition is attained more rapidly in the older retina. The data suggest a progressive stabilization of the enzyme-forming system during differentiation.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Glutamine Synthetase Activity in Human Retinal Tissue: Enzyme Activity in the Normal Retina and Cortisol Induction in Cultured Cells Derived From Retinoblastoma.
L. Reif-Lehrer (1971)
Arch Ophthalmol 86, 72-76
   Abstract »    PDF »
Glutamine Synthetase in the Normal Human Retina.
G. J. Chader, L. Saunders, and L. Reif-Lehrer (1971)
Arch Ophthalmol 85, 286-287
   Abstract »    PDF »
Induction of Glutamine Synthetase in Embryonic Retina: Its Dependence on Cell Interactions.
J. E. Morris and A. A. Moscona (1970)
Science 167, 1736-1738
   Abstract »    PDF »
Enzyme Induction by Corticosteroids in Embryonic Cells: Steroid Structure and Inductive Effect.
A. A. Moscona and R. Piddington (1967)
Science 158, 496-497
   Abstract »    PDF »
Physiological Chemistry of the Eye: A Review of Papers Published During 1964.
S. L. BONTING (1965)
Arch Ophthalmol 74, 561-578
   PDF »


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