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Science 16 April 1965:
Vol. 148. no. 3668, pp. 387 - 389
DOI: 10.1126/science.148.3668.387
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Articles

Density-Gradient Centrifugation: Non-Ideal Sedimentation and the Interaction of Major and Minor Components

Myron K. Brakke 1 and J. M. Daly 2

1 Crops Research Division, U.S. Agricultural Research Service, Nebraska Agricultural Experiment Station, Lincoln
2 Biochemistry and Nutrition Department, Nebraska Agricultural Experiment Station

A small amount of southern bean mosaic virus was contained in a narrow zone after density-gradient centrifugation, but in a much wider zone after centrifugation with a large amount of a second virus. Zone-spreading of a major component by non-ideal sedimentation in density-gradient centrifugation can cause zone spreading of a minor component that the major component overlaps.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Zonal Centrifuges and Other Separation Systems.
N. G. Anderson (1966)
Science 154, 103-112
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Science. ISSN 0036-8075 (print), 1095-9203 (online)