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Science 12 February 1965:
Vol. 147. no. 3659, pp. 740 - 741
DOI: 10.1126/science.147.3659.740
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Articles

Enzyme from Soil Bacterium Hydrolyzes Phenylcarbamate Herbicides

Philip C. Kearney 1 and Donald D. Kaufman 1

1 Crops Research Division, U.S. Department of Agriculture, Beltsville, Maryland

An enzyme preparation from Pseudomonas sp., isolated from a soil culture by an enrichment technique, liberated 3-chloroaniline from the herbicide isopropyl N-(3-chlorophenyl) carbamate. Aniline, 3-chloroaniline, and 3,4-dichloroaniline were detected when the enzyme preparation was incubated with several alkyl esters of the phenylcarbamates and chlorophenylcarbamates. No chloroaniline was detected when the 3-(p-chlorophenyl)-1,1-dimethylurea (monuron) was used as a substrate. The substrate specificity of the isolated enzyme suggests that it catalyzes the initial hydrolysis of many biologically active phenylcarbamates in soils.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Enzymatic Degradation of Chlorodiamino-s-Triazine.
J. L. Seffernick, N. Shapir, M. Schoeb, G. Johnson, M. J. Sadowsky, and L. P. Wackett (2002)
Appl. Envir. Microbiol. 68, 4672-4675
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