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Science 5 February 1965:
Vol. 147. no. 3658, pp. 615 - 617
DOI: 10.1126/science.147.3658.615

Articles

Polysomes from Yeast: Distribution of Messenger RNA and Capacity to Support Protein Synthesis in vitro

Leon Marcus 1, Roger Bretthauer 1, Harlyn Halvorson 1, and Robert Bock 2

1 Department of Bacteriology, University of Wisconsin, Madison
2 Department of Biochemistry, University of Wisconsin

Individual fractions of polysomes were isolated from yeast. Pulse labeling experiments in vivo show constant specific activity of messenger RNA in each polysome peak; this suggests a uniform density of ribosomes per unit length of messenger RNA. In the cell-free incorporating system, the amount of peptide per ribosome unit increased with the size of polysome.





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Science. ISSN 0036-8075 (print), 1095-9203 (online)