Polysomes from Yeast: Distribution of Messenger RNA and Capacity to Support Protein Synthesis in vitro
Leon Marcus 1,
Roger Bretthauer 1,
Harlyn Halvorson 1, and
Robert Bock 2
1 Department of Bacteriology, University of Wisconsin, Madison
2 Department of Biochemistry, University of Wisconsin
Individual fractions of polysomes were isolated from yeast. Pulse labeling experiments in vivo show constant specific activity of messenger RNA in each polysome peak; this suggests a uniform density of ribosomes per unit length of messenger RNA. In the cell-free incorporating system, the amount of peptide per ribosome unit increased with the size of polysome.