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Science 30 October 1964:
Vol. 146. no. 3644, pp. 659 - 661
DOI: 10.1126/science.146.3644.659
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Articles

Antibody Formation in vitro by Separated Spleen Cells: Inhibition by Actinomycin or Chloramphenicol

Sven-Erik Svehag 1

1 Department of Virology, Public Health Research Institute of the City of New York, New York 10009

The formation of 19S antibody to poliovirus by rabbit spleen cells in vitro was interrupted by adding actinomycin D (1 to 10 micrograms per milliliter) for 30 minutes or longer. Shorter treatment (5 to 15 minutes) caused partial suppression. Antibody formation was slowly renewed upon removal of the drug. These results suggest that 19S antibody formation is contingent upon DNA-dependent RNA synthesis and that the genetic messenger has an average lifetime of one-half day or less. Treatment with chloramphenicol (20 to 50 micrograms per milliliter) for 3 days caused the cessation of 19S antibody formation.

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Science. ISSN 0036-8075 (print), 1095-9203 (online)