Protein Purification by Elution Convection Electrophoresis
Samuel Raymond 1
1 Pepper Laboratory, University of Pennsylvania, Philadelphia
Purified protein components can be recovered from high-resolution gel-electrophoresis patterns by elution-convection electrophoresis. The protein components are all simultaneously eluted from the gel pattern by horizontal electrophoresis and are then simultaneously concentrated by electro-convection to recover the proteins in nearly the original sample concentration. Thirty individual protein fractions can be recovered in 3 hours from a sample containing 200 milligrams of protein.