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Science 28 August 1964:
Vol. 145. no. 3635, pp. 942 - 943
DOI: 10.1126/science.145.3635.942
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Articles

Aminoacyl Position in Aminoacyl sRNA

Calvin S. McLaughlin 1 and Vernon M. Ingram 1

1 Division of Biochemistry, Department of Biology, Massachusetts Institute of Technology, Cambridge

Chromatographic systems capable of resolving the 2'- and 3'-isomers of valyladenosine and N-acetylvalyladenosine are used to show that valyladenosine isolated from valyl-sRNA is the equilibrium mixture of 2'- and 3'-acyl isomers by the time of chromatography. Acyl migration in N-acetylvalyladenosine is shown to be a rapid, base-catalyzed reaction with a rate 40,000 times the rate of hydrolysis under equivalent conditions. Calculations indicate that the pool of free aminoacyl sRNA exists in vivo as the equilibrium mixture of the 2'- and 3'-aminoacyl isomers

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Science. ISSN 0036-8075 (print), 1095-9203 (online)