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Science 3 April 1964:
Vol. 144. no. 3614, pp. 64 - 65
DOI: 10.1126/science.144.3614.64
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Articles

Freezing and Viability of Tetrahymena pyriformis in Dimethylsulfoxide

Shuh-Wei Hwang 1, E. E. Davis 1, and M. T. Alexander 1

1 American Type Culture Collection, Rockville, Maryland 20852

Ciliated protozoa may be preserved at very low temperatures. Tetrahymena pyriformis, suspended in 10 percent dimethylsulfoxide, survived when frozen in two steps, first to -20 ° C, then transferring immediately to a temperature of -196 ° C. Motility and ability to reproduce were recovered after freezing by this method and subsequent storage for 3 months in a liquid nitrogen refrigerator.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Tetrahymena: Effect of Freezing and Subsequent Thawing on Breeding Performance.
E. M. Simon and S.-W. Hwang (1967)
Science 155, 694-696
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Science. ISSN 0036-8075 (print), 1095-9203 (online)