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Science 27 December 1963:
Vol. 142. no. 3600, pp. 1655 - 1656
DOI: 10.1126/science.142.3600.1655
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Articles

Culture of Small Barley Embryos on Defined Media

Knut Norstog 1 and Joyce E. Smith 2

1 Botany Program, University of South Florida, Tampa
2 Department of Botany, Cornell University, Ithaca, New York

Barley embryos, measuring 90 Fz in length, grew and became differentiated on a phosphate-enriched White's medium which was fortified with glutamine and alanine as major sources of organic nitrogen, and lesser amounts of five other amino acids. The pH of the medium was a critical factor in differentiation. On this medium, excised barley embryos developed more rapidly than embryos remaining in vivo, or embryos grown on media containing coconut milk.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Single Cells, Coconut Milk, and Embryogenesis in vitro.
W. Halperin (1966)
Science 153, 1287-1288
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Science. ISSN 0036-8075 (print), 1095-9203 (online)