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Science 29 November 1963:
Vol. 142. no. 3596, pp. 1183 - 1184
DOI: 10.1126/science.142.3596.1183
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Articles

Continuous Recording of Cell Number in Logarithmic and Synchronized Cultures

T. W. James 1 and Norman G. Anderson 1

1 Biology Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee

An instrument for the continuous recording of cell number has been developed and is being used to record changes in populations of logarithmic and synchronized cultures of protozoan flagellates. A Coulter cell counter is used in conjunction with a counting chamber that is fitted with a flexible polyethylene aperture (75 µ in diameter). This aperture rarely becomes blocked and appears to be self-clearing. The unit consists of a proportioning pump, a cell counter, a counting chamber, a rate meter, and a recorder.

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Science. ISSN 0036-8075 (print), 1095-9203 (online)