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Science 18 October 1963:
Vol. 142. no. 3590, pp. 389 - 390
DOI: 10.1126/science.142.3590.389
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Articles

Malate Dehydrogenase: Multiple Forms in Separated Blastomeres of Sea Urchin Embryos

Richard O. Moore 1 and Claude A. Villee 1

1 Marine Biological Laboratory, Woods Hole, Massachusetts, Department of Agricultural Biochemistry, Ohio State University, Columbus, and Department of Biological Chemistry, Harvard Medical School, Boston, Massachusetts

Sea urchin embryos at the 64-cell stage were dissociated by treatment with trypsin and separated by centrifugation on a sucrose gradient. The large blastomeres have two and the small blastomeres have three bands of L-malate dehydrogenase activity, which are separated by disk microelectrophoresis on polyacrylamide gel, whereas unfertilized eggs have five.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Malic Dehydrogenase Isozymes: Distribution in Developing Nucleate and Anucleate Halves of Sea Urchin Eggs.
G. W. Patton Jr., L. Mets, and C. A. Villee (1967)
Science 156, 400-401
   Abstract »    PDF »
D-Malate: Effects on Activity of L-Malate Dehydrogenase in Developing Sea Urchin Embryos.
R. D. Billiar, J. C. Brungard, and C. A. Villee (1964)
Science 146, 1464-1465
   Abstract »    PDF »


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