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Science 7 September 1962:
Vol. 137. no. 3532, pp. 752 - 754
DOI: 10.1126/science.137.3532.752
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Articles

Electron Spin Resonance of Nitric Oxide-Hemoglobin Complexes in Solution

K. M. Sancier 1, G. Freeman 1, and J. S. Mills 1

1 Stanford Research Institute, Menlo Park, California

The electron spin resonance spectra of solutions of nitric oxide-hemoglobin and nitric oxide-methemoglobin, and whole blood treated at room temperature with nitric oxide, all exhibit resonance with a line width of 83 gauss, a g-value of 2.03, and a spin intensity corresponding to one unpaired electron spin per heme. The minimum detectable concentration of these nitric oxide complexes in solution is 10-5M. Solutions were stable in a nitrogen atmosphere but when exposed to air in the absence of nitric oxide the spin intensity decreased with a half-life of about 5 hours. A preliminary examination of blood of rats exposed 1 and 9 days to 10 ppm of nitric oxide in air showed no electron spin resonance.

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Science. ISSN 0036-8075 (print), 1095-9203 (online)