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Science 6 May 1960:
Vol. 131. no. 3410, pp. 1376 - 1377
DOI: 10.1126/science.131.3410.1376
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Articles

New Method for Manipulation, Maintenance, and Cloning of Single Mammalian Cells in vitro

M. Aronson 1 and R. W. I. Kessel 1

1 Institute of Microbiology, Rutgers University, New Brunswick, New Jersey

Individual mammalian cells can be isolated with the aid of easily handled glass beads to which the cells have become attached. Procedures for the preparation of cell cultures on beads, and for the recognition and manipulation of beads carrying single monocytes, HeLa cells, and Detroit-98 cells are described. Data obtained with Brucella infected monocytes, illustrating the efficiency of the method, are presented.

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Science. ISSN 0036-8075 (print), 1095-9203 (online)