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Science 5 June 1959:
Vol. 129. no. 3362, pp. 1550 - 1551
DOI: 10.1126/science.129.3362.1550
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Articles

Incorporation of Unnatural Pyrimidine Bases into Deoxyribonucleic Acid of Mammalian Cells

M. L. EIDINOFF 1, L. CHEONG 1, and M. A. RICH 1

1 Division of Biophysics, Sloan-Kettering Institute for Cancer Research, New York, New York

When a mammalian cell strain was incubated with 5-iododeoxyuridine and 5-bromodeoxyuridine, DNA thymine was partially replaced by the halogen-containing pyrimidines. The extent of incorporation of the unnatural bases increased when amethopterin and hypoxanthine were added to the medium. It is thus evident that the replacement of DNA thymine by selected structural analogs, a phenomenon previously reported for bacterial systems, is applicable to cells of higher organisms.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Polydactylism in the Offspring of Mice Injected with 5-Bromodeoxyuridine.
J. A. Dipaolo (1964)
Science 145, 501-503
   Abstract »    PDF »
Incorporation of Idoxuridine (IDU) by the Rabbit Lens Epithelium in Vitro.
M. B. WHEELER, C. V. HARDING, and W. L. HUGHES (1964)
Arch Ophthalmol 71, 861-864
   Abstract »    PDF »


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